Medicineworld.org: How Blood Clots Are Formed?
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How Blood Clots Are Formed?
A model of platelets in actionGood science requires great patience. In a number of fields, ideas and theories surge ahead while the tools to test them can take decades to catch up. When Peter Richardson began talking with colleagues who were modeling blood flow through the vessels on the heart's surface, he hardly suspected that the collaboration would lead to a test of ideas he had proposed more than 30 years before.
The resulting model, described in the online edition of Proceedings of the National Academy of Sciences appearing the week of Oct. 30 to Nov. 3, could help evaluate candidate drugs aimed at preventing blood clots - a major cause of strokes, heart attacks and organ transplant rejection.
In 1970, Richardson, who had been working on the clotting problems linked to artificial organs, saw a paper describing the time course of clot formation in uninjured blood vessels. Gustav Born and his co-author, Nicola Begent, had found an odd relationship - shaped like a playground slide - between the rate of blood flow and the rate of blood clot, or thrombus, formation. As blood flow increased, the rate at which the clot grew increased rapidly, up to a point. After that point, the rate of growth declined suddenly and then gradually flattened out.
Richardson recognized that there must be two groups of processes at work - probably one chemical and one physical. The increase in clotting with flow made sense. Faster blood flow meant more platelets encountered the clot each second, so more had a chance to be captured by it. But the decrease was puzzling.
Scientists knew that ADP (adenosine diphosphate) released from injured tissue or existing thrombi could signal platelets to begin sticking together at the site of the injury. Richardson proposed that a short delay between triggering and activation could explain the decrease in aggregation with increased blood flow. "If triggering is the time when the alarm goes off," said Richardson, a professor of engineering and physiology at Brown University, "then activation is when your feet hit the floor." As blood flows faster, more platelets have already passed beyond the clot by the time they are ready for action.
Richardson published his ideas at the time, but the computing power needed to simulate more than 50,000 platelets and their individual responses to a growing clot wasn't yet available. Recently, when co-authors Igor Pivkin and George Karniadakis sought Richardson's input on a similarly detailed simulation of blood flow in arteries on the heart's surface, the team realized that the time had come to test Richardson's hypothesis.
Pivkin and Karniadakis modified their model to fit Richardson's proposal and made hundreds of runs, changing flow rate, activation time, and the strength of attraction between activated platelets. Each set of runs produced a relationship between flow and aggregation remarkably similar to what Begent and Born had found in hamsters.
While no recent experimental data set has the same detailed time course as Begent and Born's, Richardson and his colleagues have observed that their model is able to reproduce several odd features of thrombus growth that have been observed both in the laboratory and in tissue pathologies from operating rooms and morgues. At high flow rates, they find the shape of the thrombus goes from "hill-like" to "carpet-like." At very high flow rates and short activation times, they see secondary clots forming downstream from the primary site.
The model is generalized in the sense that it does not require a number of specific details, such as the concentration of fibrinogen (which binds clots together) or the length of fibrinogen strands. It doesn't need to specifically calculate concentration or diffusion of the activation factor. Yet it produces remarkably true-to-life results.
In the next few years, the team will refine the model by incorporating molecular and sub-cellular details while enhancing the representation of fluid dynamics and interactions between platelets and other cells, working toward a truly multiscale model. Richardson also has physiological experiments planned that will allow more rigorous testing and refinement of the model.
Revisiting this line of inquiry, says Richardson, may lead experimental scientists to explore drugs that could change the activation time of platelets, leading to better preventive strategies for clots, whether they result from blood vessel injury, atherosclerotic plaque or an artificial medical device.
Posted by: Scott Source
Did you know?
Good science requires great patience. In a number of fields, ideas and theories surge ahead while the tools to test them can take decades to catch up. When Peter Richardson began talking with colleagues who were modeling blood flow through the vessels on the heart's surface, he hardly suspected that the collaboration would lead to a test of ideas he had proposed more than 30 years before.
Medicineworld.org: How Blood Clots Are Formed?
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