MedicineWorld.Org
Your gateway to the world of medicine
Home
News
Cancer News
About Us
Cancer
Health Professionals
Patients and public
Contact Us
Disclaimer

Medicineworld.org: Tracking Cells In The Body

Back to research news Blogs list Cancer blog  


Subscribe To Research News RSS Feed  RSS content feed What is RSS feed?

Tracking Cells In The Body

Tracking Cells In The Body
Scientists' inability to follow the whereabouts of cells injected into the human body has long been a major drawback in developing effective medical therapies. Now, scientists at Johns Hopkins have developed a promising new technique for noninvasively tracking where living cells go after they are put into the body. The new technique, which uses genetically encoded cells producing a natural contrast that can be viewed using magnetic resonance imaging (MRI), appears much more effective than present methods used to detect injected biomaterials.

Described in the February edition of Nature Biotechnology, the method was developed by a team of scientists from Johns Hopkins' Russell H. Morgan Department of Radiology and Radiological Science, the Hopkins Institute for Cell Engineering, and the F.M. Kirby Research Center for Functional Brain Imaging at the Kennedy Krieger Institute in Baltimore.

In their study, the scientists used a synthetic gene, called a reporter gene, which was engineered to have a high proportion of the amino acid lysine, which is particularly rich in accessible hydrogen atoms. Because MRI detects energy-produced shifts in hydrogen atoms, when the "new" gene was introduced into animal cells and then "pelted" with radiofrequency waves from the MRI, it became readily visible. Using the technique as a proof of principle, the scientists were able to detect transplanted tumor cells in animal brains.

"This prototype paves the way for constructing a family of reporter genes, each with proteins tailored to have a specific radiofrequency response," says MRI researcher Assaf Gilad, Ph.D., lead author of the study.

"The specific frequencies can be processed to show up as colors in the MRI image," adds collaborator Mike McMahon, Ph.D., an assistant professor of radiology at the Johns Hopkins School of Medicine "In a way, it's the MRI equivalent of the green and red fluorescent proteins found in nature and used by labs everywhere in the world for multiple labeling of cells".

The problem with using fluorescent proteins, however, is that tissue must be removed from the body for examination under a microscope, which means that the method isn't suitable for use in patients. "In contrast," says Hopkins radiology professor Jeff Bulte, Ph.D., "MRI is noninvasive, allowing serial imaging of cells and cellular therapies with a high resolution unmatched by any other clinical whole-body imaging technique."

Current MRI contrast agents also have several disadvantages. "Their concentration becomes lower every time cells divide," says Peter van Zijl, Ph.D., founding director of the Kirby Research Center for Functional Brain Imaging, "so our ability to see them diminishes. Also, using magnetic metal allows us to detect only one type of labeled cell at a time." The new approach is not hampered by these limitations.


Posted by: Scott    Source




Did you know?
Scientists' inability to follow the whereabouts of cells injected into the human body has long been a major drawback in developing effective medical therapies. Now, scientists at Johns Hopkins have developed a promising new technique for noninvasively tracking where living cells go after they are put into the body. The new technique, which uses genetically encoded cells producing a natural contrast that can be viewed using magnetic resonance imaging (MRI), appears much more effective than present methods used to detect injected biomaterials.

Medicineworld.org: Tracking Cells In The Body

Acute bacterial meningitis| Alzheimer's disease| Carpal tunnel syndrome| Cerebral aneurysms| Cerebral palsy| Chronic fatigue syndrome| Cluster headache| Dementia| Epilepsy seizure disorders| Febrile seizures| Guillain barre syndrome| Head injury| Hydrocephalus| Neurology| Insomnia| Low backache| Mental retardation| Migraine headaches| Multiple sclerosis| Myasthenia gravis| Neurological manifestations of aids| Parkinsonism parkinson's disease| Personality disorders| Sleep disorders insomnia| Syncope| Trigeminal neuralgia| Vertigo|

Copyright statement
The contents of this web page are protected. Legal action may follow for reproduction of materials without permission.