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Medicineworld.org: Our microbes, ourselves

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Our microbes, ourselves




In terms of diversity and sheer numbers, the microbes occupying the human gut easily dwarf the billions of people inhabiting the Earth. Numbering in the tens of trillions and representing a number of thousands of distinct genetic families, this microbiome, as it's called, helps the body perform a variety of regulatory and digestive functions, a number of still poorly understood.

How this microbial mlange appears to be associated with body weight changes linked to morbid obesity is a relevant and important clinical question that has received recent attention. Now, a newly released study suggests that the composition of microbes within the gut may hold a key to one cause of obesityand the prospect of future therapy.



Our microbes, ourselves
The team's study is the first molecular survey of gut microbial diversity following surgical weight loss (gastric bypass), and has helped solidify the link between methane producing microbes and obesity. This means the drastic anatomical changes created by gastric bypass surgery appear to have profound effects on the microorganisms that inhabit the intestine. This change may be part of the reason that gastric-bypass surgery is the most effective means to treat obesity today.

Credit: Mayo Clinic


In the January 19 early online edition of the Proceedings of the National Academy of Science, scientists at Arizona State University's Biodesign Institute in collaboration with colleagues at the Mayo Clinic, Arizona, and the University of Arizona, reveal a tantalizing link between differing microbial populations in the human gut and body weight among three distinct groups: normal weight individuals, those who have undergone gastric bypass surgery, and patients suffering the condition of morbid obesitya serious, often life-threatening condition linked to diabetes, cardiovascular disease, cancer and psychosocial disorders. Obesity affects around 4 million Americans and, each year, some 300,000 die from obesity-related illness.




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The team's study is the first molecular survey of gut microbial diversity following surgical weight loss (gastric bypass), and has helped solidify the link between methane producing microbes and obesity.


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A collaboration aimed at uncovering the links between the microbial composition of the human gut and morbid obesity began when Dr. John DiBaise, a gastroenterologist at the Mayo Clinic, Arizona, got interested in both the underlying mechanisms of obesity and plausible alternatives to gastric bypass surgerystill the only reliable long-term therapy for the extremely overweight.

DiBaise turned to Bruce Rittmann, Ph.D., an environmental engineer and a member of National Academy of Engineering, whose Center for Environmental Biotechnology uses its expertise to examine microbial populations important for cleaning up pollutants and generating renewable bioenergy. Rittmann invited Rosa Krajmalnik-Brown, assistant professor of civil and environmental engineering, to collaborate and apply her microbial ecology expertise to this project. The three scientists were able to leverage seed funding from the Mayo Clinic and ASU so that they could combine their respective talents. DiBaise recruited 9 middle-aged volunteers in three groupsnormal weight, morbidly obese and following gastric bypass surgeryto participate in the study.

The research team's central hypothesis is that differing microbial populations in the gut allow the body to harvest more energy, making people more susceptible to developing obesity. These small differences can, over time, profoundly affect an individual's weight. Supporting this view is the study's confirmation that the microbial composition among obese patients appears significantly altered compared with both normal weight individuals and those who have undergone gastric bypass surgery.

A microbial managerie
To tease out the microbial human gut composition, Husen Zhang, a postdoctoral scholar working with Rittmann and Krajmalnik-Brown, used an advanced DNA sequencing technology and sophisticated ecological tools. The team examined 184,094 gene sequences of microbial 16S rRNA, a molecular structure which provides a characteristic fingerprint for microbial identification. The analysis was conducted with the assistance of University of Arizona's Rod Wing at the Bio5 Institute, using a novel sequencing technique known as 454-pyrosequencing, which allows a significantly larger number and greater diversity of gut microbia to be identified.

The group's latest findings represent the first investigation of gut microbiota from post-gastric-bypass patients to date.

By examining a specific region of the 16S rRNA gene known as V6PCR amplified from the stool samples of the 9 test subjectsthe scientists were able to classify a zoo of microorganisms, which fell into 6 broad categories, with two bacterial phyla, the bacteroidetes and firmicutes, predominanting.

The resulting composition of gut microbiota in the three gastric bypass patients differed substantially and in potentially important ways from obese and normal weight individuals. This means the drastic anatomical changes created by gastric bypass surgery appear to have profound effects on the microorganisms that inhabit the intestine. This change appears to be part of the reason that gastric-bypass surgery is the most effective means to treat obesity today.

The team's study is the first molecular survey of gut microbial diversity following surgical weight loss, and has helped solidify the link between methane producing microbes and obesity. Specifically, the microbial populations extracted from obese individuals were high in a particular microbial subgroup, hydrogen-producing bacteria known as prevotellaceae. Further, such hydrogen producers appear to coexist with hydrogen-consuming methanogens, found in abundance in obese patients, but absent in both normal weight and gastric bypass samples. Unlike the hydrogen producers, however, these methane-liberating hydrogen consumers are not bacteria. They belong instead to the third great microbial domainthe Archaea, (with Eukarya and Bacteria making up the other two).

Energy managers
During the course of digestion, calories are extracted from food and stored in fat tissue for later usea process delicately regulated by the multitude of microbial custodians. The intermediary products of the digestive process include hydrogen, carbon dioxide and several short chain fatty acids (SCFAs).

Results suggest a cooperative co-existence in obese individuals between hydrogen-producers and hydrogen consuming methanogens. Rittmann explains how this mutually reinforcing relationship, known as syntrophy, may contribute to obesity:.

"Organisms producing hydrogen and acetate create a situation like cars flooding onto the highway. The methanogens, which remove the hydrogen, are like the offramps, allowing the hydrogen cars to get off. That allows more acetate cars to get on, because some hydrogen cars are coming off the highway".

The methanogen offramps, by removing hydrogen, accelerate the efficient fermentation of otherwise indigestible plant polysaccharides and carbohydrates. The effect is to boost production of SCFAs, especially acetate, which will be taken up by the intestinal epithelium and converted to fat. The result over time appears to be increasing weight, eventually leading to obesity.

While weight regulation involves a complex interplay of genetic predisposition, exercise, eating habits, and other factors, manipulation of the gut's microflora, especially the methanogenic Archaea, may provide additional avenues for the therapy of morbid obesity.

The scientists stress that the study is preliminary, but were encouraged by the findings from their small sample. Future investigation is needed to establish the differences in composition of gut microbiota across different age groups and under varying weight-loss regimens involving diet and exercise. Nevertheless, the study's findings point to new avenues for modifying the body's energy harvesting efficiencyperhaps by manipulation of the Bacteria-Archaea nexus.


Posted by: Mark    Source




Did you know?
In terms of diversity and sheer numbers, the microbes occupying the human gut easily dwarf the billions of people inhabiting the Earth. Numbering in the tens of trillions and representing a number of thousands of distinct genetic families, this microbiome, as it's called, helps the body perform a variety of regulatory and digestive functions, a number of still poorly understood.

Medicineworld.org: Our microbes, ourselves

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